How to choose PCR boards with different skirt styles? What is the difference between white and clear PCR tubes?

AMNGENT CONSUMABLES 

What is the difference between white and transparent tubes when selecting PCR consumables? How to choose a PCR board with a variety of skirt styles? Let's give you a brief explanation of these two problems!

PCR tubes/plates are commonly available in white and transparent, and in traditional PCR experiments, there is not much difference in the use of the two, but in quantitative PCR experiments, the sensitivity and consistency of white tubes are relatively higher for qPCR instruments that require apical signal reading. Let's briefly explain why.

The core data of qPCR is the Ct value, and the fluorescence signal is the data source for the Ct value calculation, so it is critical that the fluorescence in the tube is well transmitted to the detector. The white tube wall prevents fluorescence from passing through the tube wall and refraction, thereby preventing fluorescence from transmitting to the heating module, that is, the metal hole wall where the tube is placed, and the transparent tube will cause fluorescence to be absorbed by the module or inconsistent reflection. Therefore, compared with transparent tubes, the use of white tubes can effectively avoid signal interference, so as to collect stronger and more focused fluorescence signals.

Amngent PCR tubes & plates

The skirt of a PCR plate refers to the extension around the plate. There are three common types of skirts, semi-skirts and full skirts. Unskirted can be used universally on all models, while fully skirted PCR boards are typically used in automated workstations. The skirting role is to provide stability support for the pipetting process when building reaction systems, as well as mechanical strength and grip when combined with automated workstations. Simply put, the significance of skirting is more to facilitate industrialized and automated batch processing, and the combination of robotic arms.

Semi-skirted PCR plates are usually divided into two types, Roche and ABI, which are used for these two PCR instruments.

Amngent PCR tubes & plates

Amngent PCR tubes are made of medical-grade polypropylene, with ultra-thin walls and extremely fast temperature conduction. The flat lid design is suitable for real-time quantitative PCR experiments, and the special sealing design effectively avoids the problem of bursting the lid. No DNase, RNase, no endotoxin. The working temperature range is -20°C~100°C, which can be matched with conventional brand PCR instrument and can withstand 6000G of fully supported rotor Centrifugal force.

Amngent PCR plates are also made of medical-grade polypropylene, with ultra-thin tube walls and extremely fast temperature conduction. There are many options without skirt, half skirt, and full skirt. The plate surface is flat and the hole circumference is convex, which is convenient for sealing film pasting.

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