Summary:
1. Different types of micropipettes have their own suction volume ranges. Please use the appropriate range micropipette according to the volume of the solution to be taken.2. When setting the volume, rotate from low to high, first surpass the desire......
1. Different types of micropipettes have their own suction volume ranges. Please use the appropriate range micropipette according to the volume of the solution to be taken.
2. When setting the volume, rotate from low to high, first surpass the desired set value by at least 1/3 turn, and then reverse to the set value; turn from high to low, then directly turn to the set value That's it. Do not turn the volume adjustment ring beyond the use range of Zdi or Zgao.
3. To install the tip, use the rotary installation method. Place the tip of the pipette close to the tip. While pressing down gently, the pipette in the handle rotates 180 degrees counterclockwise. Do not use excessive force to avoid damaging the pipette and affecting its extreme accuracy.
4. When transferring the liquid, the liquid to be transferred should be sucked and released 2-3 times, so that a homogeneous liquid film can be formed on the inner wall of the tip to ensure the accuracy and accuracy of the pipetting work.
5. When aspirating liquid, the tip immersed in the liquid should be at an appropriate depth, and the liquid aspiration process should be kept unchanged as much as possible. Do not immerse the micropipette body in the solution. In the process of sampling and adding samples, it should be noted that the pipette tip cannot touch other objects to avoid contamination. When aspirating general liquid, press the button with your finger to the first position, keep the micropipette as vertical as possible, immerse the micropipette in the solution, and then slowly release the button. After waiting for 1 second, lift the tip off the liquid surface and stay on the wall for 1~3 seconds to make the droplets on the outside of the tube tip slip off. Do not release the button too fast, so as to prevent the solution from rushing into the straw and corroding the piston.
When pipetting viscous or volatile liquids, it is easy to cause large volume errors. The following methods are recommended to improve the accuracy of pipetting. To aspirate high-viscosity test solution, please cut the outlet of the micropipette head with a blade or scissors first, and pre-moisten it before aspirating. Before pipetting the liquid, repeatedly pipette the liquid several times to pre-wet the tip. When suctioning or discharging liquid, Z stays for a few seconds, especially when pipetting a larger volume of liquid. Using the reverse-phase pipetting method, press to the second gear when aspirating and slowly release the control button, and part of the liquid remains in the tip. After absorbing the acid or liquid, the micro pipette should be disassembled, all parts should be rinsed clean, and then the correct combination should be restored to its original state after wiping dry.
6. When discharging the liquid, keep a certain inclination, stick the suction head to the inner wall of the container and press the button to the diyi stop point. After 1 second, press the button to the second stop point to discharge the remaining liquid. Press the button while lifting the sampler so that the tip is rubbed against the container wall to release the button. Then use the ejection function of the pipette itself to discard the tip to the recycling equipment or waste equipment, and dispose of it properly.
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