Summary:1) Basic knowledge about sample bottles:The requirements of the sample bottle are accurate volume, able to withstand a certain pressure, good sealing performance, and no adsorption to the sample. The appropriate one should be selected according to th......
1) Basic knowledge about sample bottles:
The requirements of the sample bottle are accurate volume, able to withstand a certain pressure, good sealing performance, and no adsorption to the sample. The appropriate one should be selected according to the requirements of the instrument and the condition of the sample. Sealing cover: In order to prevent the sealing gasket from adsorbing the sample components, a gasket lined with polytetrafluoroethylene or aluminum is often used. The selection depends on the analysis conditions (temperature) and the specific conditions of the sample. Routine analysis can use butyl rubber pads and trace analysis with lined silicone rubber pads. When necessary, use blank analysis to confirm that the volatiles in the gasket do not interfere with the analysis.
2) About how to choose a sample bottleFirst of all, we must understand that sample vials mainly include crimp cap vials, screw cap vials and bayonet cap vials.If you want a universal type and an acid-resistant type, you can choose a glass sample bottle;If it is used for very small injection volume, a micro-lined tube is required;If it is used for samples that are easy to stick to the wall of glass bottles and trace analysis, silanization/deactivation is required;If it is used for light-sensitive samples, a brown bottle is required;If it is used for alcohol samples or water-soluble solvents, polypropylene is required.3) From how to clean the sample bottleIn fact, when we do liquid chromatography, it is best to use disposable sample bottles, but this cost is too high, so now we use duplicate sample bottles, we have to clean them better. We can use organic reagents for cleaning as much as possible. Ethanol can be used for cleaning. If you are in a hurry, you can also use a hair dryer after cleaning with ethanol.