Summary:
Most glass bottles can be kept intact at -4°C. At room temperature, short-term storage of test samples does not require any special precautions and can be stored in glass vials containing organic solvents at temperatures as low as -80°C. However, us......
Most glass bottles can be kept intact at -4°C. At room temperature, short-term storage of test samples does not require any special precautions and can be stored in glass vials containing organic solvents at temperatures as low as -80°C. However, use caps made of polypropylene in particular.
Because these materials will become more and more brittle when the temperature is lower than 0 ℃. Here are some principles to follow when freezing glass bottles:
storage bottle
The most important thing when using it as a storage bottle is to keep it out of direct sunlight. It's best to keep it on a plastic bottle rack during storage and thawing.
The thawing process of many liquids, especially if the sample contains a lot of water, can put considerable pressure on the closed container and may cause it to rupture. Therefore, when storing liquid samples at low temperatures, the sample cannot fill the entire container.
At extremely low temperatures, snap-top vials are not recommended, and glass vials used at low temperatures should have a larger internal volume and a smaller opening. Such as 8-425 threaded vials and clear crimp top vials. A large open bottle with good results should be used. However, operating precautions must be observed, using a closed top cover or adhesive diaphragm to open the top cover.
When glass bottles are stored at low temperature for a long time, it is recommended to crimp seal or top-fasten screw caps.
Thaw cryovials
Frozen bottles must be handled with extreme care. In general, glass bottles will crack (especially brown bottles) when exposed to sudden changes in temperature. So don't put the cold bottle in a warm place and avoid hitting it. Placing the bottle on top of a metal object also increases its likelihood of breakage.
Note that the temperature of the bottle will gradually approach the temperature of the outside environment. In addition, it is best not to touch or bump it when the temperature inside the bottle is not the same as the temperature outside.
Warming frozen bottle
Glass bottles should be allowed to warm up gradually, and warm glass bottles should avoid sharp changes in temperature. It is not recommended to use a water bath, hot water tank, oven or other high heat source to warm glass bottles. It is most reasonable to thaw glass vials slowly by heat conduction at room temperature. Glass bottles whose temperature is lower than -20°C should be placed in the refrigerator at -4°C to 0°C first. This reduces the possibility of damage to the glass bottle.
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