Summary:Although the casing of the holding part of the pipette is generally made of low thermal conductivity materials, the temperature of the hand will still be transmitted to the pipette after a long time of continuous holding, and the parts inside may be ......
Although the casing of the holding part of the pipette is generally made of low thermal conductivity materials, the temperature of the hand will still be transmitted to the pipette after a long time of continuous holding, and the parts inside may be affected by the temperature. It will be deformed due to changes, thus affecting the pipetting accuracy.
Therefore, pay attention to:
First, during the operation, as long as the thumb is not required, try to use the finger hook to hang the pipette on the hand and release the palm;
Second, generally, continuous pipetting should not exceed half an hour. If the workload is heavy, you can change hands. In this way, your precious hands get some rest while minimizing the impact of hand temperature.
High Density/Low Density Samples
The accuracy values of the pipette are all based on transferring pure water. If the density of the sample is very different from the density of the water, the accuracy will be correspondingly poor. Therefore, it is necessary to find out the density of the sample before pipetting, and then adjust the range to the product of the volume to be transferred and the density.
For example, if the density of a sample is 1.2g/cm3 and you need to transfer 300ul, then you should set the volume to 360ul. This is only a rough adjustment method, and strictly requires a measuring instrument or a balance as an auxiliary tool for accurate calculation. In short, this is basically a job that requires experience.
High temperature/low temperature samples
Pay attention to three points: first, never rinse the tip before pipetting; second, change a tip every time you pipet; third, suction and discharge should be completed as soon as possible.